Comparison of a Point-of-Care Assay and a High-Complexity Assay for Detection of SARS-CoV-2 RNA.
Identifieur interne : 000679 ( Main/Exploration ); précédent : 000678; suivant : 000680Comparison of a Point-of-Care Assay and a High-Complexity Assay for Detection of SARS-CoV-2 RNA.
Auteurs : Bryan Stevens [États-Unis] ; Catherine A. Hogan [États-Unis] ; Malaya K. Sahoo [États-Unis] ; Chunhong Huang [États-Unis] ; Natasha Garamani [États-Unis] ; James Zehnder [États-Unis] ; Jason Kurzer ; Benjamin A. Pinsky [États-Unis]Source :
- The journal of applied laboratory medicine [ 2576-9456 ] ; 2020.
Descripteurs français
- KwdFr :
- ARN viral (isolement et purification), Betacoronavirus (génétique), Betacoronavirus (isolement et purification), Facteurs temps (MeSH), Humains (MeSH), Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Laboratoire automatique (instrumentation), Pandémies (MeSH), Pneumopathie virale (diagnostic), Pneumopathie virale (virologie), Pneumopathie virale (épidémiologie), Reproductibilité des résultats (MeSH), Réaction de polymérisation en chaine en temps réel (instrumentation), Réaction de polymérisation en chaine multiplex (instrumentation), Systèmes automatisés lit malade (statistiques et données numériques), Techniques de diagnostic moléculaire (instrumentation), Techniques de diagnostic moléculaire (statistiques et données numériques), Techniques de laboratoire clinique (instrumentation), Techniques de laboratoire clinique (statistiques et données numériques), Trousses de réactifs pour diagnostic (statistiques et données numériques), États-Unis (épidémiologie).
- MESH :
- diagnostic : Infections à coronavirus, Pneumopathie virale.
- génétique : Betacoronavirus.
- isolement et purification : ARN viral, Betacoronavirus, Laboratoire automatique, Réaction de polymérisation en chaine en temps réel, Réaction de polymérisation en chaine multiplex, Techniques de diagnostic moléculaire, Techniques de laboratoire clinique.
- statistiques et données numériques : Systèmes automatisés lit malade, Techniques de diagnostic moléculaire, Techniques de laboratoire clinique, Trousses de réactifs pour diagnostic.
- virologie : Infections à coronavirus, Pneumopathie virale.
- épidémiologie : Infections à coronavirus, Pneumopathie virale, États-Unis.
- Facteurs temps, Humains, Pandémies, Reproductibilité des résultats.
- Wicri :
- geographic : États-Unis.
English descriptors
- KwdEn :
- Automation, Laboratory (instrumentation), Betacoronavirus (genetics), Betacoronavirus (isolation & purification), COVID-19 (MeSH), COVID-19 Testing (MeSH), Clinical Laboratory Techniques (instrumentation), Clinical Laboratory Techniques (statistics & numerical data), Coronavirus Infections (diagnosis), Coronavirus Infections (epidemiology), Coronavirus Infections (virology), Humans (MeSH), Molecular Diagnostic Techniques (instrumentation), Molecular Diagnostic Techniques (statistics & numerical data), Multiplex Polymerase Chain Reaction (instrumentation), Pandemics (MeSH), Pneumonia, Viral (diagnosis), Pneumonia, Viral (epidemiology), Pneumonia, Viral (virology), Point-of-Care Systems (statistics & numerical data), RNA, Viral (isolation & purification), Reagent Kits, Diagnostic (statistics & numerical data), Real-Time Polymerase Chain Reaction (instrumentation), Reproducibility of Results (MeSH), SARS-CoV-2 (MeSH), Time Factors (MeSH), United States (epidemiology).
- MESH :
- chemical , isolation & purification : RNA, Viral.
- geographic , epidemiology : United States.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- epidemiology : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus.
- instrumentation : Automation, Laboratory, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Multiplex Polymerase Chain Reaction, Real-Time Polymerase Chain Reaction.
- isolation & purification : Betacoronavirus.
- statistics & numerical data : Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Point-of-Care Systems, Reagent Kits, Diagnostic.
- virology : Coronavirus Infections, Pneumonia, Viral.
- COVID-19, COVID-19 Testing, Humans, Pandemics, Reproducibility of Results, SARS-CoV-2, Time Factors.
Abstract
BACKGROUND
Numerous nucleic acid amplification assays utilizing different target genes of the SARS-CoV-2 genome have received emergency use authorization (EUA) by the United States Food and Drug Administration (FDA). Limited data are available comparing the test performance characteristics of these assays.
METHODS
A diagnostic comparison study was performed to evaluate the performance of the Cepheid Xpert Xpress SARS-CoV-2 assay compared to the Hologic Panther Fusion SARS-CoV-2 assay using clinical nasopharyngeal specimens. Agreement between the two assays was assessed by overall, positive, and negative percent agreement and Cohen's kappa coefficient.
RESULTS
A total of 104 (54 positive and 50 negative) clinical nasopharyngeal samples were tested by both assays. Using the Panther Fusion as a reference standard, the Xpert demonstrated an overall agreement of 99.0% [95% confidence interval (CI): 94.8-100], positive percent agreement of 98.1% (95% CI: 90.1-100), and a negative percent agreement of 100% (95% CI: 94.2-100). The kappa coefficient was 0.98 (95% CI: 0.94-1.0). One sample positive by the Panther Fusion with a cycle threshold (Ct) of 38.6 was found to be reproducibly negative by the Xpert assay.
CONCLUSIONS
The Cepheid Xpert Xpress SARS-CoV-2 assay provides test performance comparable to the Hologic Panther Fusion SARS-CoV-2 assay while offering laboratories rapid, on-demand testing capacity.
DOI: 10.1093/jalm/jfaa135
PubMed: 32761092
PubMed Central: PMC7454585
Affiliations:
Links toward previous steps (curation, corpus...)
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<term>Clinical Laboratory Techniques (statistics & numerical data)</term>
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<term>Coronavirus Infections (epidemiology)</term>
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<term>Molecular Diagnostic Techniques (statistics & numerical data)</term>
<term>Multiplex Polymerase Chain Reaction (instrumentation)</term>
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<term>Pneumonia, Viral (virology)</term>
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<term>Infections à coronavirus (virologie)</term>
<term>Infections à coronavirus (épidémiologie)</term>
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<term>Pneumopathie virale (virologie)</term>
<term>Pneumopathie virale (épidémiologie)</term>
<term>Reproductibilité des résultats (MeSH)</term>
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<term>Réaction de polymérisation en chaine multiplex</term>
<term>Techniques de diagnostic moléculaire</term>
<term>Techniques de laboratoire clinique</term>
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<keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Clinical Laboratory Techniques</term>
<term>Molecular Diagnostic Techniques</term>
<term>Point-of-Care Systems</term>
<term>Reagent Kits, Diagnostic</term>
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<term>Techniques de diagnostic moléculaire</term>
<term>Techniques de laboratoire clinique</term>
<term>Trousses de réactifs pour diagnostic</term>
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<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
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<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Pneumonia, Viral</term>
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<term>Time Factors</term>
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<term>Humains</term>
<term>Pandémies</term>
<term>Reproductibilité des résultats</term>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Numerous nucleic acid amplification assays utilizing different target genes of the SARS-CoV-2 genome have received emergency use authorization (EUA) by the United States Food and Drug Administration (FDA). Limited data are available comparing the test performance characteristics of these assays.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODS</b>
</p>
<p>A diagnostic comparison study was performed to evaluate the performance of the Cepheid Xpert Xpress SARS-CoV-2 assay compared to the Hologic Panther Fusion SARS-CoV-2 assay using clinical nasopharyngeal specimens. Agreement between the two assays was assessed by overall, positive, and negative percent agreement and Cohen's kappa coefficient.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>A total of 104 (54 positive and 50 negative) clinical nasopharyngeal samples were tested by both assays. Using the Panther Fusion as a reference standard, the Xpert demonstrated an overall agreement of 99.0% [95% confidence interval (CI): 94.8-100], positive percent agreement of 98.1% (95% CI: 90.1-100), and a negative percent agreement of 100% (95% CI: 94.2-100). The kappa coefficient was 0.98 (95% CI: 0.94-1.0). One sample positive by the Panther Fusion with a cycle threshold (Ct) of 38.6 was found to be reproducibly negative by the Xpert assay.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>The Cepheid Xpert Xpress SARS-CoV-2 assay provides test performance comparable to the Hologic Panther Fusion SARS-CoV-2 assay while offering laboratories rapid, on-demand testing capacity.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32761092</PMID>
<DateCompleted><Year>2020</Year>
<Month>12</Month>
<Day>01</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>12</Month>
<Day>18</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">2576-9456</ISSN>
<JournalIssue CitedMedium="Print"><Volume>5</Volume>
<Issue>6</Issue>
<PubDate><Year>2020</Year>
<Month>11</Month>
<Day>01</Day>
</PubDate>
</JournalIssue>
<Title>The journal of applied laboratory medicine</Title>
<ISOAbbreviation>J Appl Lab Med</ISOAbbreviation>
</Journal>
<ArticleTitle>Comparison of a Point-of-Care Assay and a High-Complexity Assay for Detection of SARS-CoV-2 RNA.</ArticleTitle>
<Pagination><MedlinePgn>1307-1312</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1093/jalm/jfaa135</ELocationID>
<Abstract><AbstractText Label="BACKGROUND">Numerous nucleic acid amplification assays utilizing different target genes of the SARS-CoV-2 genome have received emergency use authorization (EUA) by the United States Food and Drug Administration (FDA). Limited data are available comparing the test performance characteristics of these assays.</AbstractText>
<AbstractText Label="METHODS">A diagnostic comparison study was performed to evaluate the performance of the Cepheid Xpert Xpress SARS-CoV-2 assay compared to the Hologic Panther Fusion SARS-CoV-2 assay using clinical nasopharyngeal specimens. Agreement between the two assays was assessed by overall, positive, and negative percent agreement and Cohen's kappa coefficient.</AbstractText>
<AbstractText Label="RESULTS">A total of 104 (54 positive and 50 negative) clinical nasopharyngeal samples were tested by both assays. Using the Panther Fusion as a reference standard, the Xpert demonstrated an overall agreement of 99.0% [95% confidence interval (CI): 94.8-100], positive percent agreement of 98.1% (95% CI: 90.1-100), and a negative percent agreement of 100% (95% CI: 94.2-100). The kappa coefficient was 0.98 (95% CI: 0.94-1.0). One sample positive by the Panther Fusion with a cycle threshold (Ct) of 38.6 was found to be reproducibly negative by the Xpert assay.</AbstractText>
<AbstractText Label="CONCLUSIONS">The Cepheid Xpert Xpress SARS-CoV-2 assay provides test performance comparable to the Hologic Panther Fusion SARS-CoV-2 assay while offering laboratories rapid, on-demand testing capacity.</AbstractText>
<CopyrightInformation>© American Association for Clinical Chemistry 2020. All rights reserved. For permissions, please email: journals.permissions@oup.com.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Stevens</LastName>
<ForeName>Bryan</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Stanford University School of Medicine, Stanford, CA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Clinical Virology Laboratory, Stanford Health Care, Stanford, CA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hogan</LastName>
<ForeName>Catherine A</ForeName>
<Initials>CA</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Stanford University School of Medicine, Stanford, CA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Clinical Virology Laboratory, Stanford Health Care, Stanford, CA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sahoo</LastName>
<ForeName>Malaya K</ForeName>
<Initials>MK</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Stanford University School of Medicine, Stanford, CA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Huang</LastName>
<ForeName>ChunHong</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Stanford University School of Medicine, Stanford, CA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Garamani</LastName>
<ForeName>Natasha</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Stanford University School of Medicine, Stanford, CA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zehnder</LastName>
<ForeName>James</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Stanford University School of Medicine, Stanford, CA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kurzer</LastName>
<ForeName>Jason</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Stanford University School of Medicine, Stanford, CA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pinsky</LastName>
<ForeName>Benjamin A</ForeName>
<Initials>BA</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Stanford University School of Medicine, Stanford, CA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Clinical Virology Laboratory, Stanford Health Care, Stanford, CA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType>
<PublicationType UI="D023362">Evaluation Study</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Appl Lab Med</MedlineTA>
<NlmUniqueID>101693884</NlmUniqueID>
<ISSNLinking>2475-7241</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011933">Reagent Kits, Diagnostic</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D057205" MajorTopicYN="N">Automation, Laboratory</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000073640" MajorTopicYN="N">Betacoronavirus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000086382" MajorTopicYN="N">COVID-19</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000086742" MajorTopicYN="N">COVID-19 Testing</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019411" MajorTopicYN="N">Clinical Laboratory Techniques</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="Y">instrumentation</QualifierName>
<QualifierName UI="Q000706" MajorTopicYN="N">statistics & numerical data</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D025202" MajorTopicYN="N">Molecular Diagnostic Techniques</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="Y">instrumentation</QualifierName>
<QualifierName UI="Q000706" MajorTopicYN="N">statistics & numerical data</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060885" MajorTopicYN="N">Multiplex Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D058873" MajorTopicYN="N">Pandemics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011024" MajorTopicYN="N">Pneumonia, Viral</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019095" MajorTopicYN="N">Point-of-Care Systems</DescriptorName>
<QualifierName UI="Q000706" MajorTopicYN="N">statistics & numerical data</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011933" MajorTopicYN="N">Reagent Kits, Diagnostic</DescriptorName>
<QualifierName UI="Q000706" MajorTopicYN="N">statistics & numerical data</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000086402" MajorTopicYN="N">SARS-CoV-2</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014481" MajorTopicYN="N" Type="Geographic">United States</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">COVID-19</Keyword>
<Keyword MajorTopicYN="Y">Cepheid GeneXpert</Keyword>
<Keyword MajorTopicYN="Y">Panther Fusion</Keyword>
<Keyword MajorTopicYN="Y">SARS-CoV-2</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year>
<Month>06</Month>
<Day>08</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2020</Year>
<Month>07</Month>
<Day>15</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2020</Year>
<Month>8</Month>
<Day>8</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>12</Month>
<Day>2</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2020</Year>
<Month>8</Month>
<Day>8</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">32761092</ArticleId>
<ArticleId IdType="pii">5881637</ArticleId>
<ArticleId IdType="doi">10.1093/jalm/jfaa135</ArticleId>
<ArticleId IdType="pmc">PMC7454585</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Californie</li>
</region>
</list>
<tree><noCountry><name sortKey="Kurzer, Jason" sort="Kurzer, Jason" uniqKey="Kurzer J" first="Jason" last="Kurzer">Jason Kurzer</name>
</noCountry>
<country name="États-Unis"><region name="Californie"><name sortKey="Stevens, Bryan" sort="Stevens, Bryan" uniqKey="Stevens B" first="Bryan" last="Stevens">Bryan Stevens</name>
</region>
<name sortKey="Garamani, Natasha" sort="Garamani, Natasha" uniqKey="Garamani N" first="Natasha" last="Garamani">Natasha Garamani</name>
<name sortKey="Hogan, Catherine A" sort="Hogan, Catherine A" uniqKey="Hogan C" first="Catherine A" last="Hogan">Catherine A. Hogan</name>
<name sortKey="Hogan, Catherine A" sort="Hogan, Catherine A" uniqKey="Hogan C" first="Catherine A" last="Hogan">Catherine A. Hogan</name>
<name sortKey="Huang, Chunhong" sort="Huang, Chunhong" uniqKey="Huang C" first="Chunhong" last="Huang">Chunhong Huang</name>
<name sortKey="Pinsky, Benjamin A" sort="Pinsky, Benjamin A" uniqKey="Pinsky B" first="Benjamin A" last="Pinsky">Benjamin A. Pinsky</name>
<name sortKey="Pinsky, Benjamin A" sort="Pinsky, Benjamin A" uniqKey="Pinsky B" first="Benjamin A" last="Pinsky">Benjamin A. Pinsky</name>
<name sortKey="Pinsky, Benjamin A" sort="Pinsky, Benjamin A" uniqKey="Pinsky B" first="Benjamin A" last="Pinsky">Benjamin A. Pinsky</name>
<name sortKey="Sahoo, Malaya K" sort="Sahoo, Malaya K" uniqKey="Sahoo M" first="Malaya K" last="Sahoo">Malaya K. Sahoo</name>
<name sortKey="Stevens, Bryan" sort="Stevens, Bryan" uniqKey="Stevens B" first="Bryan" last="Stevens">Bryan Stevens</name>
<name sortKey="Zehnder, James" sort="Zehnder, James" uniqKey="Zehnder J" first="James" last="Zehnder">James Zehnder</name>
</country>
</tree>
</affiliations>
</record>
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